• 1. 
    How will advances in genetics most likely affect biotechnology?

  • Biotechnology will provide cures for all diseases in the future.
  • Biotechnology will gradually phase out of the field of medicine.
  • Biotechnology will create new genes that will make certain diseases disappear from the human genome.
  • Biotechnology will enable better prediction and treatment of various diseases.
  • 2. 
    Which has the Human Genome Project been useful in determining?

  • the gender of an individual
  • the frequency of crossing-over during meiosis
  • whether a fetus has Down Syndrome
  • whether individuals could carry genes for a certain condition
  • 3. 
    Maggie is a transgenic goat. How does being transgenic make Maggie different from non-transgenic goats?

  • Maggie has DNA from both of her parents.
  • Maggie has DNA that winds around proteins.
  • Maggie has DNA implanted from another organism
  • Maggie has DNA that uses a different coding system.
  • 4. 
    Genetically engineered bacteria are currently being used to produce certain human proteins. Which best explains the advantage associated with this process?

  • The genetically engineered proteins can be used to produce human clones.
  • The genetically engineered proteins have been found to cure most cancers.
  • The genetically engineered proteins are less harmful than normal human proteins.
  • The genetically engineered proteins can be produced in large quantities with less cost.
  • 5. 
    Which would be the benefit of a transgenic crop?

  • decreased drought tolerance
  • decreased disease resistance
  • increased resistance to pests
  • increased demands for nutrients
  • 6. 
    How does each person’s DNA differ?

  • The bonds in the DNA are all different.
  • There are different orders of nitrogenous bases.
  • The phosphates in the DNA are different.
  • There are different kinds of sugars in the nucleotides.
  • 7. 
    Which are uses for DNA fingerprinting?

  • identifying related individuals and treating genetic disorders
  • identifying genes for genetic disorders and producing vaccines
  • matching individual DNA samples and identifying related individuals
  • determining the number of human genes and forming transgenic organisms
  • 8. 
    How might the applications of biotechnology have the potential to negatively impact individuals?

  • using genetically-engineered enzymes in laundry detergents
  • producing genetically-engineered bacteria for creating human drugs
  • producing genetically-engineered foods without having research on their safety
  • using genetically-engineered enzymes for biofuels in an attempt to replace fossil fuels
  • 9. 
    Which substance is cut by restriction enzymes?

  • DNA
  • carbohydrates
  • proteins
  • lipids
  • 10. 
    Which procedure is the final step in the production of a transgenic organism?

  • Recombinant DNA is transferred to a bacterial cell
  • A specific gene in a DNA sequence is identified.
  • The DNA segment is combined into a plasmid.
  • The DNA segment to be inserted is isolated.
  • 11. 
    Which procedure is the first step in the production of a transgenic organism?

  • Recombinant DNA is transferred to a bacterial cell.
  • A specific gene in a DNA sequence is identified.
  • The DNA segment is combined into a plasmid.
  • The DNA segment to be inserted is isolated.
  • 12. 
    Which best describes recombinant DNA?

  • DNA that has been deactivated by enzymes
  • DNA found in organelles other than the nucleus
  • inserted genes from one organism into another to direct it to produce a protein
  • genes developed when an egg nucleus and a sperm nucleus fuse to form a zygote
  • 13. 
    Which of these procedures involves using DNA fingerprinting as a common practice to identify an individual?

  • determining possible future weight and height of a child
  • determining the parents of a baby that may have been switched at birth
  • using blood, skin, or other tissues to solve crimes
  • identifying a decomposed body or part of a body
  • 14. 
    What do all organisms have in common?

  • They use DNA and RNA to pass on information.
  • They are all eukaryotes.
  • They are all prokaryotes.
  • They are genetically identical.
  • 15. 
    How can gel electrophoresis be used for identification of individuals when over 99 percent of human DNA is the same?

  • Each person’s DNA will band in different colors in the gel tray
  • The 99 percent of the DNA that is the same will reflect UV light differently
  • It focuses only on the small regions of the DNA which are known to vary among individuals.
  • Experts can determine the different genes by reading the genetic alphabet displayed in the gel.
  • 16. 
    Which step of bacterial transformation occurs after a gene of interest has been isolated?

  • removing the plasmid from the bacterium
  • inserting the plasmid into a bacterium
  • inserting the gene of the parent generation into the plasmid
  • cutting the plasmid with restriction enzymes
  • 17. 
    Which would provide the least genetic evidence of a crime?

  • fingerprints
  • saliva
  • hair
  • blood
  • 18. 
    Which is a transgenic organism?

  • a mutation in an animal cell
  • an animal cell with no mutation
  • bacteria with antibiotic resistance
  • a plant with bacterial DNA
  • 19. 
    Two people believe they are related. Which would be the best technique to determine if they are related?

  • testing blood types
  • comparing DNA
  • examining karyotypes
  • testing for genetic disorders
  • 20. 
    What is the primary purpose of using restriction enzymes in gel electrophoresis?

  • It restricts the number of base pairs that can be tested in a sample.
  • It makes the testing simpler by moving the strands into the gel faster.
  • It allows the DNA strands to be charged so the electrical current can send the strands into the gel.
  • It allows the strands of DNA to be cut into various lengths for testing.
  • 21. 
    Which technique would most likely be used by forensic scientists?

  • gene cloning
  • gene therapy
  • DNA fingerprinting
  • karyotyping
  • 22. 
    Gel electrophoresis separates DNA according to size. Which is true regarding movement of the DNA?

  • The longest pieces of DNA will move the least distance.
  • The longest pieces of DNA will move the greatest distance.
  • The shortest pieces of DNA line up along the middle.
  • The shortest pieces of DNA line up an equal distance from the middle
  • 23. 
    Several procedures involved in the creation of a transgenic organism are listed. 1. A specific gene is identified in DNA. 2. The DNA fragment is placed in a plasmid. 3. The DNA fragment of interest is isolated. 4. The plasmid is transferred to a bacterial cell. 5. A plasmid is cut by a restriction enzyme. Which selection represents the correct order of events in creating a transgenic organism?

  • 1, 3, 2, 4, 5
  • 3, 5, 2, 4, 1
  • 1, 3, 5, 2, 4
  • 3, 1, 4, 2, 5
  • 24. 
    Scientists have produced plants that are resistant to herbicides. Which application of biotechnology is most likely being used?

  • genetic cloning
  • recombinant DNA
  • DNA fingerprinting
  • genetic engineering
  • 25. 
    Which steps are in the correct order to create insulin-producing bacteria?

  • cut bacterial plasmid with restriction enzyme, insert human insulin-producing gene, seal with ligase enzyme
  • seal with ligase enzyme, cut bacterial plasmid with restriction enzyme, insert human insulin-producing gene
  • cut bacterial plasmid with restriction enzyme, seal with ligase enzyme, insert human insulin-producing gene
  • insert human insulin-producing gene, cut bacterial plasmid with restriction enzyme, seal with ligase enzyme
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