Quiz  /  PCR Gate & PSU MCQ Questions With Answers

  • 1. 
    At what temperature does annealing of primers take place in PCR?

  • 50 - 65oC
  • 94oC
  • 37oC
  • 72oC

  • 2. 
    Which of the following statements are true regarding PCR

  • Automated PCR machines are called thermal cyclers
  • A thermostable DNA Polymerase is required
  • Billion copies of desired DNA can be synthesized from microgram quantities of DNA
  • All of these

  • 3. 
    The PCR technique was developed by

  • Karry Mullis
  • Kohler
  • Milstein
  • Altman

  • 4. 
    What is the best definition for "primer"?

  • An oligonucleotide of unknown sequence that is used to amplify the target gene.
  • An oligonucleotide of known sequence that is used to amplify the target gene.
  • An oligonucleotide of known sequence that is annealed to the target gene, allowing Taq polymerase to bind.
  • A section of DNA on the target gene that is the binding site for Taq polymerase.

  • 5. 
    Reverse transcription PCR uses

  • mRNA as a template to form
  • RNA as a template to form a DNA
  • DNA as a template to form ssDNA
  • all of these

  • 6. 
    Which of the following is true regarding PCR

  • Denaturation involves heating at 90-98 degree C
  • Annealing involves binding of primer between 40-60 degree C
  • Primer extension occurs at 72 degree C
  • All of these

  • 7. 
    At what temperature does denaturation take place in PCR?

  • 50 - 65oC
  • 94oC
  • 37oC
  • 72oC

  • 8. 
    The basic requirements of PCR reaction include

  • DNA segment to be amplified
  • Two oligonucleotide primers
  • a heat stable DNA polymerase
  • all of these

  • 9. 
    At what temperature does extension of the target gene take place in PCR?

  • 50 - 65oC
  • 94oC
  • 37oC
  • 72oC

  • 10. 
    PCR is a

  • DNA Sequencing technique
  • DNA Amplification technique
  • DNA Degradation technique
  • All of these

  • 11. 
    If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?

  • 2
  • 4
  • 8
  • 32

  • 12. 
    A technique for making millions of copies of a specific region of DNA.

  • DNA ligase
  • restriction enzymes
  • gel electrophoresis
  • polymerase chain reaction

  • 13. 
    All of the following are thermostable polymerases except

  • Taq Polymerase
  • Vent Polymerase
  • DNA Polymerase III
  • pfu Polymerase

  • 14. 
    Denaturation involves

  • heating between 40-60 C
  • heating between 90-98 C
  • heating between 70-72 C
  • None of these

  • 15. 
    What is the function of DNA Polymerase?

  • attach primers to target sequence
  • separate the DNA
  • attach nucleotides to the target sequence
  • separate the fragments according to size

  • 16. 
    What is the name of the enzyme used to extend the target gene?

  • Tac polymerase
  • Taq polymerase
  • DNA polymerase
  • Yak polymerase

  • 17. 
    Thermus aquaticus is the source of

  • Primase enzyme
  • Taq Polymerase
  • Vent Polymerase
  • Both 2 and 3

  • 18. 
    The first step in PCR is

  • Primer annealing
  • Denaturation
  • Primer extension
  • None of these

  • 19. 
    If the primer sequence is TCG, what section of DNA will it anneal?

  • AGC
  • CAT
  • GAC
  • ATC

  • 20. 
    Why must annealing of primers take place at between 50 - 65oC?

  • To prevent Taq polymerase from denaturing, allowing it to catalyse the binding of primers to the target gene.
  • To ensure that primer annealing happens quickly - higher rate of reaction as temperature is high.
  • To allow the formation of hydrogen bonds between the primer and target gene.
  • To prevent the formation of hydrogen bonds between the primer and target gene.
Report Question
warning
access_time
  Time